<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Yi-Tian Fu</style></author><author><style face="normal" font="default" size="100%">Yu Nie</style></author><author><style face="normal" font="default" size="100%">De-Yong Duan</style></author><author><style face="normal" font="default" size="100%">Guo-Hua Liu</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Variation of mitochondrial minichromosome composition in Hoplopleura lice (Phthiraptera: Hoplopleuridae) from rats</style></title><secondary-title><style face="normal" font="default" size="100%">Parasites &amp; Vectors</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Fragmented mt genome</style></keyword><keyword><style  face="normal" font="default" size="100%">Phylogenetic analyses</style></keyword><keyword><style  face="normal" font="default" size="100%">Rat louse</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2020</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Oct-06-2020</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04381-y</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">9 pp</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&lt;jats sec=&quot;&quot;&gt;&lt;jats title=&quot;&quot;&gt;&lt;strong&gt;Background&lt;/strong&gt;&lt;/jats&gt;&lt;strong&gt; &lt;/strong&gt;&lt;jats p=&quot;&quot;&gt;The family Hoplopleuridae contains at least 183 species of blood-sucking lice, which widely parasitize both mice and rats. Fragmented mitochondrial (mt) genomes have been reported in two rat lice (&lt;jats italic=&quot;&quot;&gt;Hoplopleura kitti&lt;/jats&gt; and &lt;jats italic=&quot;&quot;&gt;H. akanezumi&lt;/jats&gt;) from this family, but some minichromosomes were unidentified in their mt genomes.&lt;/jats&gt;&amp;nbsp; &lt;/jats&gt;&lt;jats sec=&quot;&quot;&gt;&lt;jats title=&quot;&quot;&gt;&lt;/jats&gt;&lt;/jats&gt;&lt;/p&gt;
&lt;p&gt;&lt;jats sec=&quot;&quot;&gt;&lt;jats title=&quot;&quot;&gt;&lt;strong&gt;Methods&lt;/strong&gt;&lt;/jats&gt;&lt;strong&gt; &lt;/strong&gt;&lt;jats p=&quot;&quot;&gt;We sequenced the mt genome of the rat louse &lt;jats italic=&quot;&quot;&gt;Hoplopleura&lt;/jats&gt; sp. with an Illumina platform and compared its mt genome organization with &lt;jats italic=&quot;&quot;&gt;H. kitti&lt;/jats&gt; and &lt;jats italic=&quot;&quot;&gt;H. akanezumi&lt;/jats&gt;.&lt;/jats&gt; &lt;/jats&gt;&lt;/p&gt;
&lt;p&gt;&lt;jats sec=&quot;&quot;&gt;&lt;/jats&gt;&lt;jats sec=&quot;&quot;&gt; &lt;jats title=&quot;&quot;&gt;&lt;strong&gt;Results&lt;/strong&gt;&lt;/jats&gt; &lt;jats p=&quot;&quot;&gt;Fragmented mt genome of the rat louse &lt;jats italic=&quot;&quot;&gt;Hoplopleura&lt;/jats&gt; sp. contains 37 genes which are on 12 circular mt minichromosomes. Each mt minichromosome is 1.8&amp;ndash;2.7&amp;nbsp;kb long and contains 1&amp;ndash;5 genes and one large non-coding region. The gene content and arrangement of mt minichromosomes of &lt;jats italic=&quot;&quot;&gt;Hoplopleura&lt;/jats&gt; sp. (&lt;jats italic=&quot;&quot;&gt;n&lt;/jats&gt;&amp;thinsp;=&amp;thinsp;3) and &lt;jats italic=&quot;&quot;&gt;H. kitti&lt;/jats&gt; (&lt;jats italic=&quot;&quot;&gt;n&lt;/jats&gt;&amp;thinsp;=&amp;thinsp;3) are different from those in &lt;jats italic=&quot;&quot;&gt;H. akanezumi&lt;/jats&gt; (&lt;jats italic=&quot;&quot;&gt;n&lt;/jats&gt;&amp;thinsp;=&amp;thinsp;3). Phylogenetic analyses based on the deduced amino acid sequences of the eight protein-coding genes showed that the &lt;jats italic=&quot;&quot;&gt;Hoplopleura&lt;/jats&gt; sp. was more closely related to &lt;jats italic=&quot;&quot;&gt;H. akanezumi&lt;/jats&gt; than to &lt;jats italic=&quot;&quot;&gt;H. kitti&lt;/jats&gt;, and then they formed a monophyletic group.&lt;/jats&gt; &lt;/jats&gt;&lt;jats sec=&quot;&quot;&gt; &lt;jats title=&quot;&quot;&gt;&lt;/jats&gt;&lt;/jats&gt;&lt;/p&gt;
&lt;p&gt;&lt;jats sec=&quot;&quot;&gt;&lt;jats title=&quot;&quot;&gt;&lt;strong&gt;Conclusions&lt;/strong&gt;&lt;/jats&gt; &lt;jats p=&quot;&quot;&gt;Comparison among the three rat lice revealed variation in the composition of mt minichromosomes within the genus &lt;jats italic=&quot;&quot;&gt;Hoplopleura&lt;/jats&gt;. &lt;jats italic=&quot;&quot;&gt;Hoplopleura&lt;/jats&gt; sp. is the first species from the family Hoplopleuridae for which a complete fragmented mt genome has been sequenced. The new data provide useful genetic markers for studying the population genetics, molecular systematics and phylogenetics of blood-sucking lice.&lt;/jats&gt; &lt;/jats&gt;&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Preprint version see https://www.researchsquare.com/article/rs-42350/v1 and the title of preprint version is&lt;/strong&gt; &amp;quot;&lt;strong&gt;Variation in mitochondrial minichromosome composition among Hoplopleura lice (Phthiraptera: Hoplopleuridae) from rats&amp;quot;&lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">506</style></issue></record></records></xml>