@article {95527, title = {Detecci{\'o}n de Rickettsia spp. en ectopar{\'a}sitos de animales dom{\'e}sticos y silvestres de la Reserva Natural Privada Cerro Chucant{\'\i} y comunidades aleda{\~n}as, Panam{\'a}, 2007-2010}, journal = {Biom{\'e}dica: revista del Instituto Nacional de Salud}, volume = {32}, year = {2012}, month = {Jun-30-2012}, pages = {189-195}, abstract = {

Introducci\ón. Los ectopar\ásitos son los principales vectores de rickettsiosis. En Panam\á se tienen escasos datos sobre los artr\ópodos que pudieran considerarse vectores o reservorios.

Objetivos. Presentar datos sobre la presencia de Rickettsia spp. en ectopar\ásitos de fauna silvestre y animales dom\ésticos en la Reserva Natural Privada Cerro Chucant\í y poblados vecinos.

Materiales y m\étodos. Se revisaron 9 personas, 95 mam\íferos dom\ésticos y 48 silvestres. Los animales dom\ésticos se examinaron con anuencia del propietario, mientras que la fauna silvestre se captur\ó con trampas Sherman y Tomahawk. Se extrajeron 21 especies de ectopar\ásitos: pulgas, piojos, garrapatas y otros \ácaros, los cuales se preservaron en etanol al 95 \%. Se extrajo material gen\ético de garrapatas y pulgas para ser analizado por t\écnicas moleculares en la detecci\ón de Rickettsia spp.

Resultados. Se practicaron 425 reacciones de PCR, de las cuales, 270 resultaron negativas y 155 positivas. De las positivas, 86 amplificaron para el gen gltA (55 \% de las positivas); de estos tambi\én amplificaron 41 (26 \%) para ompA. Se encontr\ó material gen\ético de Rickettsia amblyommii, en garrapatas de caballos (Amblyomma cajennense, Dermacentor nitens), de perros (Rhipicephalus sanguineus) y ninfas de Amblyomma recolectadas en el bosque. Adem\ás, se detect\ó ADN de R. felis en pulgas Ctenocephalides felis de perros.

Conclusiones. Se pudo detectar la presencia de R. amblyommii y R. felis en garrapatas y pulgas de animales dom\ésticos de los poblados cercanos a Cerro Chucant\í, aun cuando no se pudo encontrar material gen\ético de Rickettsia en ectopar\ásitos de la fauna silvestre.

Palabras clave: Rickettsia, vectores de enfermedades, animales dom\ésticos, animales salvajes, biolog\ía molecular, Panam\á

English title: Detection of Rickettsia in ectoparasites of wild and domestic mammals from the Cerro Chucanti
private reserve and from neighboring towns, Panam\á, 2007-2010

Introduction. Ectoparasites are the main vectors of rickettsiosis. In Panama, however, limited data are available concerning the arthropod species that serve as vectors or reservoirs.

Objectives. Data are presented concerning the presence of Rickettsia in ectoparasites of wildlife and domestic animals in the Cerro Chucant\í private nature reserve and in neighboring villages.

Materials and methods. Nine humans, 95 domestic mammals and 48 wild mammals were examined. Twenty-one species of ectoparasites were obtained, including fleas, lice, ticks and mites. These were preserved in 95\% ethanol. Later, the DNA was extracted from the ticks and fleas and analyzed by molecular techniques to detect presence of Rickettsia.

Results. Of a total of 425 PCR reactions, 270 were positive for Rickettsia and 155 negative. Among the positive samples, 86 PCR amplified for the gltA gene (55\% of positives) and 41 of these also amplified the ompA gene. DNA of Rickettsiaamblyommii was found in horses ticks (Amblyomma cajennense, Dermacentor nitens), dogs ticks (Rhipicephalus sanguineus) and free living nymphs in the forest. Additionally, DNA of R. felis was found in fleas from dogs Ctenocephalides felis.

Conclusions. The presence of R. amblyommii and R. felis was detected in ticks and fleas of domestic animals in villages near Cerro Chucanti; however no Rickettsia DNA was found in ectoparasites of non-domestic wildlife.

}, keywords = {disease vectors; animals, domestic; animals, Molecular Biology, Panam{\'a}., Rickettsia, wild; domestic and wild animals}, issn = {0120-4157}, doi = {10.7705/biomedica.v32i2.390}, url = {https://revistabiomedica.org/index.php/biomedica/article/view/390}, author = {Sergio Berm{\'u}dez and Roberto Miranda and Yamitzel Zald{\'\i}var and Publio Gonz{\'a}lez and Guido Berguido and Diomedes Trejos and Juan M. Pascale and Labruna, Marcelo B.} }