@article {96169, title = {Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: an emerging tool for studying the vectors of human infectious diseases}, journal = {Future Microbiology}, volume = {16}, year = {2021}, month = {Mar-01-2021}, pages = {323-340}, abstract = {

Arthropod vectors have historically been identified morphologically, and more recently using molecular biology methods. However, both of these methods are time-consuming and require specific expertise and equipment. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, which has revolutionized the routine identification of microorganisms in clinical microbiology laboratories, was recently successfully applied to the identification of arthropod vectors. Since then, the robustness of this identification technique has been confirmed, extended to a large panel of arthropod vectors, and assessed for detecting blood feeding behavior and identifying the infection status in regard to certain pathogenic agents. In this study, we summarize the state-of-the-art of matrix-assisted laser desorption ionization time-of-flight mass spectrometry applied to the identification of arthropod vectors (ticks, mosquitoes, phlebotomine sand-flies, fleas, triatomines, lice and Culicoides), their trophic preferences and their ability to discriminate between infection statuses.

}, keywords = {arthropod, entomology, MALDI-TOF MS, mosquitos, ticks, vector}, issn = {1746-0913, 1746-0921}, doi = {10.2217/fmb-2020-0145}, url = {https://www.futuremedicine.com/doi/10.2217/fmb-2020-0145}, author = {Jacques Sevestre and Adama Zan Diarra and Maureen Laroche and Lionel Almeras and Parola,Phillipe} } @article {95942, title = {Identification of Lice Stored in Alcohol Using MALDI-TOF MS}, journal = {Journal of Medical Entomology}, volume = {58}, year = {2020}, month = {Dec-21-2020}, pages = {1126-1133}, abstract = {

Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9\% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4\% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.

R\ésum\é

Les poux posent des probl\èmes majeurs de sant\é publique et v\ét\érinaire, avec des cons\équences \économiques. Leur identification est essentielle et n\écessite le d\éveloppement d\’une strat\égie innovante. Le MALDI-TOF MS a r\écemment \ét\é propos\é comme un outil rapide, peu co\ûteux et pr\écis pour l\’identification des arthropodes. L\’alcool est l\’une des m\éthodes de stockage les plus fr\équemment utilis\ées et permet de conserver les \échantillons pendant de longues p\ériodes \à temp\érature ambiante. Plusieurs \études r\écentes ont rapport\é que l\’alcool modifie les profils prot\éiques r\ésultant de l\’analyse de la SEP. Apr\ès des \études pr\éliminaires sur les poux congel\és, le but de cette recherche \était d\’\évaluer l\’influence de la conservation de l\’alcool sur la pr\écision de l\’identification des poux par la SEP MALDI-TOF. \À cette fin, des poux conserv\és dans l\’alcool pendant des p\ériodes variables ont \ét\é soumis \à l\’analyse de la SEP et les protocoles de pr\éparation des \échantillons ont \ét\é optimis\és. La reproductibilit\é et la sp\écificit\é des spectres de MS obtenus sur ces arthropodes nous ont permis de mettre en \œuvre la base de donn\ées des spectres de MS de r\éf\érence (DB) avec les profils prot\éiques de sept esp\èces de poux stock\és dans l\’alcool. Des tests en aveugle ont r\év\él\é une identification correcte de 93,9\% de Pediculus humanus corporis (Linnaeus, 1758) et de 98,4\% des autres esp\èces de poux collect\ées sur le terrain. Cette \étude a d\émontr\é que le MALDI-TOF MS pouvait \être utilis\é avec succ\ès pour l\’identification des poux stock\és dans l\’alcool pendant diff\érentes dur\ées.

Attached pdf file is preprint copy

}, keywords = {identification, lice, MALDI-TOF MS, storage method}, issn = {0022-2585, 1938-2928}, doi = {10.1093/jme/tjaa266}, url = {https://academic.oup.com/jme/article-abstract/58/3/1126/6042599}, author = {Hanene Benyahia and Basma Ouarti and Adama Zan Diarra and Mehdi Boucheikhchoukh and Mohamed Nadir Meguini and Makhlouf Behidji and Ahmed Benakhla and Parola,Phillipe and Lionel Almeras} }