@conference {96346, title = {The Effect of Insecticides on GABA Receptors of Human Louse Pediculus humanus (poster)}, booktitle = {15th The international conference of parasitology (ICOPA}, year = {2022}, month = {Aug 2022}, address = {Copenhagen - Denmark}, abstract = {

Poster at the Conference

}, url = {https://www.researchgate.net/publication/363310671_The_Effect_of_Insecticides_on_GABA_Receptors_of_Human_Louse_Pediculus_humanus}, author = {Omar Hashim and Claude L. Charvet and Toubate, Berthine and Aimun A. E. Ahmed and C{\'e}dric Neveu and Isabelle Dimier-Poisson and Fran{\c c}oise Debierre-Grockiego and Catherine Dupuy} } @conference {96345, title = {Molecular Cloning, Functional Characterization and Spatiotemporal Expression of GABA Receptor Subunits from Human Body Louse Pediculus humanus humanus}, booktitle = {8th European Congress of Pharmacology (EPHAR 2021)}, year = {2021}, month = {Dec-2021}, address = {Prague, Czech Republic}, abstract = {

A conference presentation

Abstract: Human body louse Pediculus humanus humanus is a cosmopolitan obligatory blood feeding ectoparasite, a known vector of Riketsia brawazaki, Borellia recurrentis and Bartonella quintana transmitting epidemic typhus, recurrent fever and the louse borne relapsing fever respectively. GABA receptors are traditional targets for parasiticides and insecticides, four subunits of GABA were characterized in insects; RDL, LCCH3, GRD and GC8916. The commonly used pediculicides were either resisted by emerging strains or prohibited due to high toxicity to man and environment. The homopentameric receptor constituted by PhhRDL was already described, in this article, we cloned and characterized three variants of PhhGRD, one variant of Phh8916 and one variant of PhhLCCH3, the functional expression in xenopus oocytes and the pharmacology of receptors constituted by these GABA subunits were assayed using two electrode voltage clamp(TEVC). To our knowledge, this is the first functional expression of 8916 in insects. Furthermore the relative expressions of PhhGRD, Phh8916, PhhLCCH3 and PhhRDL were predicted by quantitative real time PCR(RT-qPCR). These findings provided valuable additions to our knowledge of the complex nature of GABA receptor in human body louse that could help in understanding the resistance pattern to commonly used pediculicides and to develop new effective and selective molecules.

}, url = {https://www.researchgate.net/publication/362191070_Molecular_Cloning_Functional_Characterization_and_Spatiotemporal_Expression_of_GABA_Receptor_Subunits_from_Human_Body_Louse_Pediculus_humanus_humanus}, author = {Omar Hashim and Claude L. Charvet and Toubate, Berthine and Aimun A. E. Ahmed and C{\'e}dric Neveu and Isabelle Dimier-Poisson and Fran{\c c}oise Debierre-Grockiego and Catherine Dupuy} }