@article {46434, title = {Antigenic characterization of rat louse Polyplax spinulosa}, journal = {Folia Parasitologica (Praha)}, volume = {37}, year = {1990}, note = {LR: 20031114; JID: 0065750; 0 (Antigens); 0 (Proteins); ppublish}, month = {1990}, pages = {275 - 278}, address = {CZECHOSLOVAKIA}, abstract = {Number and relative molecular weights of proteins in whole body homogenates of the louse, Polyplax spinulosa were determined using SDS-PAGE. 13 protein components from the total of 28 bands were defined as Con A binding glycoproteins. In immunoblotting sera of lice infested laboratory rats of BN strain specifically recognized 3 major protein components with r.m.w. of 77,000, 105,000 and 230,000 Da and from 6 to 8 minor protein components with r.m.w. of 31,000-180,000 Da.}, keywords = {animals, Antigens/chemistry/immunology, Chromatography, electrophoresis, Immunoblotting, Lice Infestations/immunology, Lice/immunology, Molecular Weight, protein, rat}, isbn = {0015-5683}, author = {Volf,P. and Grubhoffer,L. and Matha,V.} } @article {40187, title = {Localization of the major immunogen and other glycoproteins of the louse Polyplax spinulosa}, journal = {International Journal for Parasitology}, volume = {24}, year = {1994}, month = {1994}, pages = {1005 - 1010}, abstract = {A 31-kDa immunogenic glycoprotein of Polyplax spinulosa was localized on louse sections using indirect immunofluorescence with specific antibodies of louse-infested rats and using fluorescein-labelled lectins. Antibodies of infested rats reacted specifically with midgut epithelium and semi-digested content of the gut. They did not bind to other louse tissues including salivary glands. The lectins concanavalin A and wheat germ agglutinin bound strongly to the immunogen, the gut and its semidigested contents. Electrophoretic and blotting analysis showed the presence of a 31-kDa glycoprotein component reacting with specific rat antibodies, concanavalin A and wheat germ agglutinin in louse faeces. The result indicates that the 31-kDa immunogen is secreted by the midgut epithelium into the gut lumen and faeces. We suggest that rats become immunized by scratching the lice faeces into the skin.}, keywords = {antibody response, antigen, antigens, glycoprotein, guinea pigs, immunization, Infestation, louse, Phthiraptera, Polyplax, protein, rat, serum}, author = {Volf,P.} }