@article {96621, title = {Contribution to the knowledge of Neotrichodectes (Nasuicola) pallidus (Piaget, 1880) (Phthiraptera: Trichodectidae)}, journal = {Veterinary Parasitology: Regional Studies and Reports}, volume = {41}, year = {2023}, month = {Jun-01-2023}, pages = {6 pp}, abstract = {

Highlights

The species in the genus Neotrichodectes (Phthiraptera: Ischnocera) infest carnivores. Neotrichodectes (Nasuicola) pallidus (Piaget, 1880), which has been primarily found parasitizing Procyonidae mammals, has been recorded in ring-tailed coatis (Nasua nasua) in the Brazilian states of Minas Gerais, Pernambuco, Santa Catarina, Rio Grande do Sul and Pernambuco. We report a new record of N. pallidus in coatis in the state of Mato Grosso do Sul, central- western Brazil, using morphological (Light and Scanning Electronic Microscopy) and molecular approaches (PCR, sequencing and phylogenetic analysis). Coatis were sampled in two peri-urban areas of Campo Grande city, Mato Grosso do Sul state, Brazil, between March 2018 and March 2019, as well as in November 2021. Lice were collected and examined under light and Scanning Electron Microscopy. DNA was also extracted from nymphs and adults and submitted to PCR assays based on the 18S rRNA and cox-1 genes for molecular characterization. One hundred and one coatis were sampled from 2018 to 2019 and 20 coatis in 2021 [when the intensity of infestation (II) was not accessed]. Twenty-six coatis (26/101\–25.7\%) were infested with at least one louse, with a total of 59 lice collected in 2018\–2019. The II ranged from one to seven lice (mean 2.2 \± SD 1.7). The louse species was confirmed based on the following morphological characteristics: female gonapophyses rounded with the setae along anterior region but not in the medial margin; the male genitalia with a parameral arch not extending beyond the endometrial plate. The same ornamentation was observed on the abdomen of the females, males, and nymphs. The nymphs and the eggs were described in detail for the first time. The obtained 18S rRNA and cox1 sequences from N. pallidus clustered in a clade with other sequences of Ischnocera species. In the present study, a new record of the louse N. pallidus in central-western Brazil was provided, along with new insights into the morphological features of this species, with the first morphology contribution of nymphal and eggs stages.

}, keywords = {8S rRNA, Cox-1, louse, Phylogeny, SEM}, issn = {2405-9390}, doi = {10.1016/j.vprsr.2023.100877}, url = {https://www.sciencedirect.com/science/article/abs/pii/S2405939023000473}, author = {L{\'\i}via Perles and Ricardo Bassini-Silva and Fernando C Jacinavicius and Barreto, Wanessa Teixeira Gomes and de Macedo, Gabriel Carvalho and Santos, Filipe Martins and Herrera, Heitor Miraglia and Machado, Rosangela Zacarias and Darci Moraes Barros-Battesti and Andr{\'e}, Marcos Rog{\'e}rio} } @article {96343, title = {Molecular detection of hemotropic Mycoplasma and Bartonella species in lice from sheep and goats of Mexico}, journal = {Veterinary Parasitology: Regional Studies and Reports}, volume = {44}, year = {2023}, month = {Sep-01-2023}, pages = {8 pp}, abstract = {

he knowledge of lice associated with small ruminants, especially sheep and goats, is scarce. In Mexico, there are historical reports of six species of chewing and sucking lice associated with Capra hircus and Ovis canadensis. However, the reports did not analyze the ecology of the infestations or the presence of potentially pathogenic bacteria. For this reason, the objectives of this study were i) to identify the species of lice associated with sheep and goats in three states of the Mexican Republic, ii) to characterize the infestations, and iii) to identify the presence of bacterial pathogens. From October 2019 to August 2021, six ranches with sheep and goats were sampled in the states of Hidalgo and Veracruz. Hosts were visually inspected, and lice were retrieved with forceps. The specimens were sexed and identified using morphological taxonomic keys. DNA extraction was performed individually, and a fragment of the cytochrome oxidase subunit 1 gene (COI) was amplified for the molecular identification of the specimens. Subsequently, Anaplasma, Bartonella, Ehrlichia, Mycoplasma, and Rickettsia were molecularly detected. Additionally, the infestations were characterized by calculating the prevalence and mean abundances. We collected 563 specimens of three species, Bovicola caprae, Bovicola ovis, and Linognathus africanus. The highest infestation levels were recorded for B. ovis (66.7\%; 4.4) from Veracruz. Additionally, two Bartonella species were detected: Bartonella mellophagi in B. ovis and Bartonella capreoli in L. africanus. In contrast, Mycoplasma ovis was detected exclusively in one pool of B. ovis. This study provides new bacterial-ectoparasite associations and highlights the possible role of these neglected ectoparasites as vectors in the populations of sheep and goats from Mexico.

}, keywords = {Ecological characterization, ectoparasites, Hemoparasites, small ruminants, Veterinary parasitology}, issn = {2405-9390}, doi = {10.1016/j.vprsr.2023.100921}, url = {https://www.sciencedirect.com/science/article/abs/pii/S2405939023000916}, author = {Gerardo G. Ballados-Gonz{\'a}lez and Jos{\'e} M. Mart{\'\i}nez-Hern{\'a}ndez and Perla B. Mart{\'\i}nez-Rodr{\'\i}guez and Jannete Gamboa-Prieto and Sa{\'u}l Gonz{\'a}lez-Guzm{\'a}n and Vladimir Paredes-Cervantes and Estefania Grostieta and Ingeborg Becker and Mariel Aguilar-Dom{\'\i}nguez and Rafael F. C. Vieira and Anabel Cruz-Romero and S{\'a}nchez-Montes, Sokani} }