02913nas a2200241 4500008004100000022001400041245014900055210006900204260001600273300001000289490000700299520213200306653001602438653002102454653002102475653000902496653001402505100002002519700002002539700002002559700002002579856007202599 2022 eng d a1664-302X00aLightella neohaematopini: A new lineage of highly reduced endosymbionts coevolving with chipmunk lice of the genus Neohaematopinus0 aiLightella neohaematopinii A new lineage of highly reduced endos cAug-01-2022 a16 pp0 v133 a

Sucking lice (Anoplura) are known to have established symbiotic associations multiple times with different groups of bacteria as diverse as Enterobacteriales, Legionellales, and Neisseriales. This diversity, together with absence of a common coevolving symbiont (such as Buchnera, in aphids), indicates that sucking lice underwent a series of symbiont acquisitions, losses, and replacements. To better understand evolution and significance of louse symbionts, genomic and phylogenetic data are needed from a broader taxonomic diversity of lice and their symbiotic bacteria. In this study, we extend the known spectrum of the louse symbionts with a new lineage associated with Neohaematopinus pacificus, a louse species that commonly parasitizes North American chipmunks. The recent coevolutionary analysis showed that rather than a single species, these lice form a cluster of unique phylogenetic lineages specific to separate chipmunk species (or group of closely related species). Using metagenomic assemblies, we show that the lice harbor a bacterium which mirrors their phylogeny and displays traits typical for obligate mutualists. Phylogenetic analyses place this bacterium within Enterobacteriaceae on a long branch related to another louse symbiont, “Candidatus Puchtella pedicinophila.” We propose for this symbiotic lineage the name “Candidatus Lightella neohaematopini.” Based on the reconstruction of metabolic pathways, we suggest that like other louse symbionts, L. neohaematopini provides its host with at least some B vitamins. In addition, several samples harbored another symbiotic bacterium phylogenetically affiliated with the Neisseriales-related symbionts described previously from the lice Polyplax serrata and Hoplopleura acanthopus. Characterizing these bacteria further extend the known diversity of the symbiotic associations in lice and show unique complexity and dynamics of the system.

10acoevolution10agenome evolution10ainsect symbionts10alice10asymbiosis1 aŘíhová, Jana1 aBell, Kayce, C.1 aNováková, Eva1 aHypša, Václav uhttps://www.frontiersin.org/articles/10.3389/fmicb.2022.900312/full02046nas a2200277 4500008004100000022001400041245016200055210006900217260001600286300001200302490000800314520103000322653000901352653003201361653001301393653001601406653001901422653001801441100002201459700002001481700002101501700002001522700002601542700002501568856017501593 2022 eng d a0022-339500aSucking Lice (Phthiraptera: Anoplura) Parasitizing Mongolian Rodents with the Description of a New Species of Hoplopleura from Mountain Voles (Alticola spp.)0 aSucking Lice Phthiraptera Anoplura Parasitizing Mongolian Rodent cAug-04-2022 a353-3650 v1083 a

The sucking louse fauna associated with Mongolian mammals is inadequately known. We provide a list of 25 species of sucking lice recorded from Mongolian rodents including previously published records, and new records of specimens collected during an expedition to northwestern Mongolia in 2015. Hoplopleura inagakii Ono and Hasegawa and Polyplax cricetulis Chin are newly recorded from Mongolia and 2 new host associations in Mongolia are recorded for Hoplopleura acanthopus (Burmeister). We describe Hoplopleura altaiensis n. sp., from the Gobi Altai mountain vole, Alticola barakshin Bannikov (type host) with an additional specimen from Alticola strelzowi (Kastchenko) (Strelzow's mountain vole). Both sexes of the new species are illustrated with scanning electron micrographs and line drawings. We note small morphological differences in the shape of the female subgenital plate between specimens prepared for scanning electron microscopy versus those prepared for light microscopy following DNA extraction.

10aAsia10aHoplopleura altaiensis n.sp10aMongolia10anew species10aParasitic lice10aSmall mammals1 aDurden, Lance, A.1 aRobinson, Chase1 aCook, Joseph, A.1 aBell, Kayce, C.1 aNyamsuren, Batsaikhan1 aGreiman, Stephen, E. uhttps://bioone.org/journals/journal-of-parasitology/volume-108/issue-4/22-2/Sucking-Lice-Phthiraptera--Anoplura-Parasitizing-Mongolian-Rodents-with-the/10.1645/22-2.short