02125nas a2200325 4500008004100000022001400041245010200055210006900157260001600226300000900242490000600251520118000257653002801437653001401465653001301479653001401492653001301506653001801519100001901537700002801556700002601584700002201610700002201632700002401654700001901678700002001697700002201717700001701739856004301756 2023 eng d a2414-636600aFirst Detection of Acinetobacter baumannii in Pediculus humanus capitis from Latin America0 aFirst Detection of iAcinetobacter baumanniii in Pediculus humanu cJun-29-2023 a8 pp0 v83 a
Background:The human head louse, Pediculus humanus capitis, is a cosmopolitan blood‑sucking ectoparasite affect‑ing mostly schoolchildren in both developed and developing countries. In Honduras, chemical pediculicides are the first line of treatment, with permethrin as their main active ingredient. Despite the extended use of these products, there is currently no research investigating insecticide resistance in Honduran head lice. In head lice, the most com‑mon mechanism is knockdown resistance (kdr), which is the result of two point mutations and the associated amino acid substitutions, T917I and L920F, within the voltage‑sensitive sodium channel (VSSC).
Methods:Genomic DNA was extracted from 83 head lice collected in the localities of San Buenaventura and La Hicaca, Honduras. Polymerase chain reaction (PCR) was used to amplify a 332‑bp fragment of the VSSC gene that contains a site affected by C/T mutation which results in a T917I amino acid substitution on each human head louse genomic DNA fragments.
Results:The C/T non‑synonymous mutation which results in the T917I kdr amino acid substitution was detected in both head lice populations at frequencies ranging between 0.45–0.5. Globally, the frequency of this substitution was 0.47. Of these, 5 (6.1%) were homozygous susceptible and 78 (93.9%) were heterozygotes. The kdr‑resistant homozy‑gote (RR) was not detected in the studied populations. Thus, 93.9% of the head lice collected in Honduras harbored only one T917I allele. Exact test for the Hardy‑Weinberg equilibrium for both localities showed that genotype frequen‑cies differed significantly from expectation. In addition, San Buenaventura and La Hicaca populations had an inbreed‑ing coefficient (Fis) < 0, suggesting an excess of heterozygotes.
Conclusions:To our knowledge, this is the first study showing the presence of the C/T mutation responsible of the T917I kdr allele associated with pyrethroid resistance in P. h. capitis from Honduras. The PCR‑restriction fragment length polymorphism (RFLP) employed here has demonstrated to be a reliable, economic, and reproducible assay that can be used to accurately genotype individual head lice for the mutation encoding the resistance‑conferring T917I amino acid substitution. This highlights the necessity of proactive resistance management programmes designed to detect pyrethroid mutations before they become established within populations of head lice.
10aHonduras10aInsecticide resistance10akdr10aPyrethroid10aVoltage‑sensitive sodium channel1 aLarkin, Kelsey1 aRodríguez, Carol1 aJamani, Shabana1 aFronza, Georgina1 aRoca-Acevedo, Gonzalo1 aSanchez, Ana1 aToloza, Ariel, Ceferino uhttps://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04183-202734nas a2200337 4500008004100000022004200041245015000083210006900233260001600302300001400318490000900332520171300341653001102054653000802065653001502073653001302088653001302101653001602114653001302130653000902143653001502152653001902167653001402186100002402200700002102224700001902245700002702264700002502291700001902316856006102335 2018 eng d a1175-5326 (print), 1175-5334 (online)00aNew species and additional data on the chewing louse genus Myrsidea (Phthiraptera: Menoponidae) from wild Neotropical Passeriformes (Aves)0 aNew species and additional data on the chewing louse genus iMyrs cOct-05-2018 a401 - 4310 v44183 aTwenty-four species of chewing lice of the genus Myrsidea Waterston, 1915 (Phthiraptera: Menoponidae) from Neotrop- ical Suboscines (Passeriformes: Formicariidae, Furnariidae, Pipridae, Thamnophilidae, Tityridae, Tyrannidae) are record- ed and discussed. They include: eight new species which are described and illustrated (Myrsidea capeki new species ex Chiroxiphia caudata; Myrsidea leptopogoni new species ex Leptopogon superciliaris; Myrsidea leucophthalmi new spe- cies ex Automolus leucophthalmus; Myrsidea pachyramphi new species ex Pachyramphus polychopterus; Myrsidea phi- lydori new species ex Philydor rufum; Myrsidea pyriglenae new species ex Pyriglena leucoptera; Myrsidea scleruri new species ex Sclerurus scansor and Myrsidea zuzanae new species ex Furnarius rufus), as well as nine previously known species with additional data on intraspecific morphological variability, host associations and geographical distribution (Myrsidea barbati Price, Hellenthal & Dalgleish, 2005; Myrsidea dalgleishi Valim, Price & Johnson, 2011; Myrsidea fla- viventris Price, Hellenthal & Dalgleish, 2005; Myrsidea klimesi Sychra, 2006; Myrsidea meyi Valim, Price & Johnson, 2011; Myrsidea oleaginei Price, Hellenthal & Dalgleish, 2005; Myrsidea olivacei Price, Hellenthal & Dalgleish, 2005; Myrsidea pitangi Price, Hellenthal & Dalgleish, 2005 and Myrsidea spellmani Price, Johnson & Dalgleish, 2008b). Seven further species are recorded at genus level only due to lack of adequate material. A 379 bp portion of the mitochondrial cytochrome oxidase I (COI) gene was sequenced from seven species in order to assess relative genetic divergences among Myrsidea populations.
10aBrazil10aCOI10aCosta Rica10aHonduras10anew data10anew species10aParaguay10aPeru10aSuboscines10aThamnophilidae10aTityridae1 aKOLENCIK, STANISLAV1 aSychra, Oldřich1 aPapoušek, Ivo1 aKuabara, Kamila, M. D.1 aValim, Michel, Paiva1 aLiterák, Ivan uhttps://biotaxa.org/Zootaxa/article/viewFile/38713/3306902159nas a2200301 4500008004100000245018500041210006900226260001800295300001400313490000900327520116000336653001701496653001701513653001701530653001301547653004801560653000901608653003201617653001601649653001301665653000901678653001501687100002401702700002101726700001901747700001901766856007201785 2017 eng d00aWhere are the species limits? Morphology versus genetics in Neotropical chewing lice of the genus Myrsidea (Phthiraptera: Menoponidae), with description of three new species0 aWhere are the species limits Morphology versus genetics in Neotr c26 Sept. 2017 a161 - 1790 v43243 aEleven species of lice of the genus Myrsidea Waterston, 1915 (Phthiraptera: Menoponidae) from passerine birds (Passer- iformes: Cardinalidae, Fringillidae, Thraupidae) in the Neotropical Region are recorded and discussed. They include three new species with hosts in the family Thraupidae from Paraguay, which are described and illustrated: Myrsidea flaveolae new species ex Sicalis flaveola, Myrsidea habiae new species ex Habia rubica, and Myrsidea sayacae new species ex Thraupis sayaca. Five other previously known Myrsidea species are included with new data on intraspecific morpholog- ical variability, hosts associations and geographical distribution, as follows: Myrsidea coronatae, M. lightae, M. rufi, M. seminuda, and M. violaceae. Three further species are recorded at genus level only due to lack of adequate material. A 379 bp portion of the mitochondrial cytochrome oxidase I (COI) gene was sequenced in order to assess relative genetic divergence among Myrsidea populations. The application of intra- and interspecific sequence divergences as predictor of species boundaries in the taxonomy of this megadiverse genus is discussed.
10aCardinalidae10aFringillidae10agenetic data10aHonduras10aintraspecific and interspecific divergences10alice10anew host-louse associations10anew species10aParaguay10aPeru10aThraupidae1 aKOLENCIK, STANISLAV1 aSychra, Oldřich1 aPapoušek, Ivo1 aLiterák, Ivan uhttp://www.mapress.com/j/zt/article/viewFile/zootaxa.4324.1.9/1249601352nas a2200253 4500008004100000022001400041245013200055210006900187260001600256300001400272490000900286520056200295653001700857653001500874653001300889653003200902653001300934653001500947653001900962100002000981700002101001700001901022856005701041 2016 eng d a1175-532600aRedescriptions and new host records of chewing lice of the genus Ricinus (Phthiraptera: Ricinidae) from the Neotropical Region0 aRedescriptions and new host records of chewing lice of the genus cJul-08-2017 a179 - 1890 v41543 aTwo species of the chewing louse genus Ricinus are redescribed and illustrated: Ricinus dalgleishi Nelson, 1972 from Helmitheros vermivorum (Gmelin, 1789), a new host-louse association, and Ricinus tanagraephilus Eichler, 1956 from Euphonia laniirostris d'Orbigny & Lafresnaye, 1837. Also, new host-louse associations are recorded for Ricinus vireoen- sis Nelson, 1972 from Vireo pallens Salvin, 1863, and for females of an unidentified species of Ricinus sp. from Corytho- pis delalandi (Lesson, 1831), which are described and illustrated.
10aChewing lice10aCosta Rica10aHonduras10anew host-louse associations10aParaguay10aPasserines10aredescriptions1 aVALAN, MIROSLAV1 aSychra, Oldřich1 aLiterák, Ivan uhttp://biotaxa.org/Zootaxa/issue/view/zootaxa.4154.2