04368nas a2200241 4500008004100000245010500041210006900146260001200215300001000227490000700237520363500244653000903879653001703888653001503905100001803920700002103938700001703959700002803976700002004004700001904024700002104043856006204064 2020 eng d00aDevelopment of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals0 aDevelopment of MALDITOF mass spectrometry for the identification c04-2020 a14 pp0 v273 a

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, including Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus, Menacanthus stramineus, Menopon gallinae, Chelopistes meleagridis, Goniocotes gallinae, Goniodes gigas, Lipeurus caponis and laboratory reared Pediculus humanus corporis. Good quality spectra were obtained for 305 samples. Spectral analysis revealed intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. A blind test of 248 specimens was performed against the in-lab database upgraded with new spectra and validated using molecular tools. With identification percentages ranging from 76% to 100% alongside high identification scores (mean = 2.115), this study proposes MALDI-TOF MS as an effective tool for discriminating lice species.

French title: Développement de la spectrométrie de masse MALDI-TOF MS pour l’identification de poux isolés d’animaux de ferme

Résumé

La Spectrométrie de Masse à Temps de Vol par Désorption/Ionisation Laser Assistée après Matrice est maintenant utilisée pour l’identification rapide des microorganismes isolés à partir d’échantillons cliniques et a récemment été appliquée avec succès pour l’identification des arthropodes. Dans cette étude, cet outil protéomique a été utilisé pour identifier les poux prélevés sur le bétail et la volaille en Algérie. Les spectres MALDI-TOF MS de 408 spécimens adultes ont été mesurés pour 14 espèces, dont Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus, Menacanthus stramineus, Menopon gallinae, Chelopistes meleagridis, Goniocotes gallinae, Goniodes gigas, Lipeurus caponis et Pediculus humanus corporis élevé en laboratoire. Des spectres de bonne qualité ont été obtenus pour 305 échantillons. L’analyse spectrale a révélé une reproductibilité intra-espèce et une spécificité inter-espèces qui concordaient avec la classification morphologique. Un test à l’aveugle de 248 échantillons a été effectué par rapport à la base de données de notre laboratoire mise à niveau avec de nouveaux spectres et validée à l’aide d’outils moléculaires. Avec des pourcentages d’identification allant de 76 à 100 % et des scores d’identification élevés (moyenne : 2,115), cette étude propose MALDI-TOF MS comme un outil efficace pour distinguer les espèces de poux.

 

 

10alice10aMALDI-TOF MS10aMallophaga1 aOuarti, Basma1 aLaroche, Maureen1 aRighi, Souad1 aMeguini, Mohamed, Nadir1 aBenakhla, Ahmed1 aRaoult, Didier1 aParola, Phillipe uhttps://www.parasite-journal.org/10.1051/parasite/202002603264nas a2200253 4500008004100000022001300041245007900054210006900133260002100202300001400223490000700237520252000244653001002764653002202774653000902796653002602805653001402831100002802845700001702873700002002890700002002910700002002930856006002950 2018 eng d a0972898800aInventory of lice of mammals and farmyard chicken in North-eastern Algeria0 aInventory of lice of mammals and farmyard chicken in Northeaster conline March 201 a386 - 3960 v113 a

Background and Aim: Lice are permanent ectoparasites, extremely specific to their hosts. Their great importance in veterinary medicine remain significant, they can cause their direct pathogenic actions like irritability, dermatitis, anemia, decreased weight gain, and milk production. The purpose of this work was to made the first time an inventory of mammalian lice in North-eastern Algeria.


Materials and Methods: Our survey of lice infestation was conducted on several animal species from five provinces of North-eastern Algeria. A total of 57 cattle, 83 sheep, 77 goats, 111 wild boars, and 63 farmyard chickens were examined. The collection of lice was carried out much more in mammals and chickens during the winter period. Lice were collected either manually or using brushing and kept in flasks containing 70% ethanol. The identification of lice was achieved in the laboratory using a binocular loupe.


Results: Concerning cattle, 63% and 27% of those examined subjects from Souk-Ahras and Guelma study areas, respectively, were carriers of lice. Damalinia bovis was the louse most frequently found on cattle in these two regions. Three other species were identified in Souk-Ahras: Haematopinus eurysternus (25%), Linognathus vituli (10%), and Solenopotes capillatus (5%). Regarding sheep, 39% and 24% of examined animals in Souk-Ahras and Guelma, were carrying lice. Damalinia ovis was the most frequently encountered lice on sheep in both regions. Linognathus ovillus also was identified in Souk-Ahras, representing 0.3% of the collected lice. Concerning goats, 53% and 30% of examined animals in Souk-Ahras and Guelma, were parasitized of lice. Two species of lice were found: Damalinia caprae and Linognathus africanus. For farmyard chickens, 69% and 100% of the farmyard chicken in Souk-Ahras and Mila were parasitized by lice, respectively. Menopon gallinae was the most frequently encountered louse in farmyard chicken in both regions. Eight other species were identified in Mila and four other species only in Souk-Ahras. Finally, 25% and 28% of the wild boars in Annaba and El Tarf were parasitized by lice, respectively. Haematopinus suis was the only species found on wild boars in both regions.

Conclusion: These results are to be taken into account for lice control schemes and louse-borne diseases. Keywords: boars, farmyard chickens, lice, North-eastern Algeria, ruminants.

10aboars10afarmyard chickens10alice10aNorth-eastern Algeria10aruminants1 aMeguini, Mohamed, Nadir1 aRighi, Souad1 aZeroual, Faycal1 aSaidani, Khelaf1 aBenakhla, Ahmed uhttp://www.veterinaryworld.org/Vol.11/March-2018/21.pdf