01989nas a2200229 4500008004100000022002500041245009100066210006900157260001600226300001200242490000700254520131900261653001201580653002201592653001701614653001501631100001601646700001701662700002701679700002001706856003301726 2021 eng d a1849-1170, 0350-714900aPrevalence of ectoparasites in free-range backyard chicken flocks in northeast Algeria0 aPrevalence of ectoparasites in freerange backyard chicken flocks cJun-07-2021 a693-7020 v523 a
Résumé
Les poux posent des problèmes majeurs de santé publique et vétérinaire, avec des conséquences économiques. Leur identification est essentielle et nécessite le développement d’une stratégie innovante. Le MALDI-TOF MS a récemment été proposé comme un outil rapide, peu coûteux et précis pour l’identification des arthropodes. L’alcool est l’une des méthodes de stockage les plus fréquemment utilisées et permet de conserver les échantillons pendant de longues périodes à température ambiante. Plusieurs études récentes ont rapporté que l’alcool modifie les profils protéiques résultant de l’analyse de la SEP. Après des études préliminaires sur les poux congelés, le but de cette recherche était d’évaluer l’influence de la conservation de l’alcool sur la précision de l’identification des poux par la SEP MALDI-TOF. À cette fin, des poux conservés dans l’alcool pendant des périodes variables ont été soumis à l’analyse de la SEP et les protocoles de préparation des échantillons ont été optimisés. La reproductibilité et la spécificité des spectres de MS obtenus sur ces arthropodes nous ont permis de mettre en œuvre la base de données des spectres de MS de référence (DB) avec les profils protéiques de sept espèces de poux stockés dans l’alcool. Des tests en aveugle ont révélé une identification correcte de 93,9% de Pediculus humanus corporis (Linnaeus, 1758) et de 98,4% des autres espèces de poux collectées sur le terrain. Cette étude a démontré que le MALDI-TOF MS pouvait être utilisé avec succès pour l’identification des poux stockés dans l’alcool pendant différentes durées.
Attached pdf file is preprint copy
10aidentification10alice10aMALDI-TOF MS10astorage method1 aBenyahia, Hanene1 aOuarti, Basma1 aDiarra, Adama, Zan1 aBoucheikhchoukh, Mehdi1 aMeguini, Mohamed, Nadir1 aBehidji, Makhlouf1 aBenakhla, Ahmed1 aParola, Phillipe1 aAlmeras, Lionel uhttps://academic.oup.com/jme/article-abstract/58/3/1126/604259904368nas a2200241 4500008004100000245010500041210006900146260001200215300001000227490000700237520363500244653000903879653001703888653001503905100001803920700002103938700001703959700002803976700002004004700001904024700002104043856006204064 2020 eng d00aDevelopment of MALDI-TOF mass spectrometry for the identification of lice isolated from farm animals0 aDevelopment of MALDITOF mass spectrometry for the identification c04-2020 a14 pp0 v273 aMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is now routinely used for the rapid identification of microorganisms isolated from clinical samples and has been recently successfully applied to the identification of arthropods. In the present study, this proteomics tool was used to identify lice collected from livestock and poultry in Algeria. The MALDI-TOF MS spectra of 408 adult specimens were measured for 14 species, including Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus, Menacanthus stramineus, Menopon gallinae, Chelopistes meleagridis, Goniocotes gallinae, Goniodes gigas, Lipeurus caponis and laboratory reared Pediculus humanus corporis. Good quality spectra were obtained for 305 samples. Spectral analysis revealed intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. A blind test of 248 specimens was performed against the in-lab database upgraded with new spectra and validated using molecular tools. With identification percentages ranging from 76% to 100% alongside high identification scores (mean = 2.115), this study proposes MALDI-TOF MS as an effective tool for discriminating lice species.
French title: Développement de la spectrométrie de masse MALDI-TOF MS pour l’identification de poux isolés d’animaux de ferme
Résumé
La Spectrométrie de Masse à Temps de Vol par Désorption/Ionisation Laser Assistée après Matrice est maintenant utilisée pour l’identification rapide des microorganismes isolés à partir d’échantillons cliniques et a récemment été appliquée avec succès pour l’identification des arthropodes. Dans cette étude, cet outil protéomique a été utilisé pour identifier les poux prélevés sur le bétail et la volaille en Algérie. Les spectres MALDI-TOF MS de 408 spécimens adultes ont été mesurés pour 14 espèces, dont Bovicola bovis, B. ovis, B. caprae, Haematopinus eurysternus, Linognathus africanus, L. vituli, Solenopotes capillatus, Menacanthus stramineus, Menopon gallinae, Chelopistes meleagridis, Goniocotes gallinae, Goniodes gigas, Lipeurus caponis et Pediculus humanus corporis élevé en laboratoire. Des spectres de bonne qualité ont été obtenus pour 305 échantillons. L’analyse spectrale a révélé une reproductibilité intra-espèce et une spécificité inter-espèces qui concordaient avec la classification morphologique. Un test à l’aveugle de 248 échantillons a été effectué par rapport à la base de données de notre laboratoire mise à niveau avec de nouveaux spectres et validée à l’aide d’outils moléculaires. Avec des pourcentages d’identification allant de 76 à 100 % et des scores d’identification élevés (moyenne : 2,115), cette étude propose MALDI-TOF MS comme un outil efficace pour distinguer les espèces de poux.
10alice10aMALDI-TOF MS10aMallophaga1 aOuarti, Basma1 aLaroche, Maureen1 aRighi, Souad1 aMeguini, Mohamed, Nadir1 aBenakhla, Ahmed1 aRaoult, Didier1 aParola, Phillipe uhttps://www.parasite-journal.org/10.1051/parasite/202002601406nas a2200205 4500008004100000245013800041210006900179520073300248653001300981653000900994653001501003653000901018653001601027100002001043700002001063700002001083700001901103700002001122856005801142 2014 eng d00aPrevalence and seasonal variation of mallophagan species (Phthiraptera) in free-range chickens from rural localities of oran, Algeria0 aPrevalence and seasonal variation of mallophagan species Phthira3 a
A total of 192 free-range chickens was randomly collected from rural localities in the region of Oran (Algeria) and examined to detect the presence of Mallophagan species and their prevalence, mean intensity and seasonal variation. Eight species were recorded, Chelopistes meleagridis, Cuclotogaster heterographus, Goniocotes gallinae, Goniodes dissimilis, Goniodes gigas, Lipeurus caponis, Menacanthus cornutus and Menopon gallinae. Goniocotes gallinae was the most prevalent lice identified (95,31%). The infection rate was higher in males compared to female chickens with a significant difference (P<0,05). The total number of Mallophagan species encountered was higher in winter season than in the other seasons.
10achickens10alice10aMallophaga10aOran10aTraditional1 aMedjouel, Ilyes1 aBenakhla, Ahmed1 aSenouci, Kheira1 aDjelil, Hanene1 aMatallah, Fouzi uhttp://sjournals.com/index.php/SJAs/article/view/120501635nas a2200253 4500008004100000022001300041245016600054210006900220260000900289300001400298490000700312520083400319653001201153653001701165653001301182653001701195653001001212100002001222700002001242700002001262700001901282700002001301856006001321 2013 eng d a1682835600aPrevalence and Distribution of Chewing Lice (Phthiraptera) in Free Range Chickens from the Traditional Rearing System in the Algerian North East, Area of El-Tarf0 aPrevalence and Distribution of Chewing Lice Phthiraptera in Free c2013 a721 - 7250 v123 aA study was carried out on 144 local chickens (Gallus gallus domesticus), from rural area of El-Tarf to determine the prevalence of chewing lice (Phthiraptera). The overall prevalence rate was 100% in the El- Tarf poultry. Nine different species of chewing lice were identified during this study: Menopon gallinae, Goniocotes gallinae, Lipeurus caponis, Goniodes dissimilis, Goniodes gigas, Menacanthus stramineus, Cuclotogaster heterographus, Menacanthus cornutus and Menacanthus pallidulus. Menopon gallinae (97.2%) was the most prevalent lice identified. The mean intensity of lice in the area of El-Tarf was 172.5 per chicken. Menopon gallinae was the species which has the highest mean intensity of infestation (83.2%). The distribution of nine chewing lice on poultry and their most preferred sites was determined.
10aAlgeria10aChewing lice10achickens10aPhthiraptera10arural1 aMedjouel, Ilyes1 aBenakhla, Ahmed1 aSenouci, Kheira1 aDjelil, Hanene1 aMatallah, Fouzi uhttp://www.scialert.net/abstract/?doi=ijps.2013.721.72503264nas a2200253 4500008004100000022001300041245007900054210006900133260002100202300001400223490000700237520252000244653001002764653002202774653000902796653002602805653001402831100002802845700001702873700002002890700002002910700002002930856006002950 2018 eng d a0972898800aInventory of lice of mammals and farmyard chicken in North-eastern Algeria0 aInventory of lice of mammals and farmyard chicken in Northeaster conline March 201 a386 - 3960 v113 aBackground and Aim: Lice are permanent ectoparasites, extremely specific to their hosts. Their great importance in veterinary medicine remain significant, they can cause their direct pathogenic actions like irritability, dermatitis, anemia, decreased weight gain, and milk production. The purpose of this work was to made the first time an inventory of mammalian lice in North-eastern Algeria.
Materials and Methods: Our survey of lice infestation was conducted on several animal species from five provinces of North-eastern Algeria. A total of 57 cattle, 83 sheep, 77 goats, 111 wild boars, and 63 farmyard chickens were examined. The collection of lice was carried out much more in mammals and chickens during the winter period. Lice were collected either manually or using brushing and kept in flasks containing 70% ethanol. The identification of lice was achieved in the laboratory using a binocular loupe.
Results: Concerning cattle, 63% and 27% of those examined subjects from Souk-Ahras and Guelma study areas, respectively, were carriers of lice. Damalinia bovis was the louse most frequently found on cattle in these two regions. Three other species were identified in Souk-Ahras: Haematopinus eurysternus (25%), Linognathus vituli (10%), and Solenopotes capillatus (5%). Regarding sheep, 39% and 24% of examined animals in Souk-Ahras and Guelma, were carrying lice. Damalinia ovis was the most frequently encountered lice on sheep in both regions. Linognathus ovillus also was identified in Souk-Ahras, representing 0.3% of the collected lice. Concerning goats, 53% and 30% of examined animals in Souk-Ahras and Guelma, were parasitized of lice. Two species of lice were found: Damalinia caprae and Linognathus africanus. For farmyard chickens, 69% and 100% of the farmyard chicken in Souk-Ahras and Mila were parasitized by lice, respectively. Menopon gallinae was the most frequently encountered louse in farmyard chicken in both regions. Eight other species were identified in Mila and four other species only in Souk-Ahras. Finally, 25% and 28% of the wild boars in Annaba and El Tarf were parasitized by lice, respectively. Haematopinus suis was the only species found on wild boars in both regions.
Conclusion: These results are to be taken into account for lice control schemes and louse-borne diseases. Keywords: boars, farmyard chickens, lice, North-eastern Algeria, ruminants.
10aboars10afarmyard chickens10alice10aNorth-eastern Algeria10aruminants1 aMeguini, Mohamed, Nadir1 aRighi, Souad1 aZeroual, Faycal1 aSaidani, Khelaf1 aBenakhla, Ahmed uhttp://www.veterinaryworld.org/Vol.11/March-2018/21.pdf