01989nas a2200229 4500008004100000022002500041245009100066210006900157260001600226300001200242490000700254520131900261653001201580653002201592653001701614653001501631100001601646700001701662700002701679700002001706856003301726 2021 eng d a1849-1170, 0350-714900aPrevalence of ectoparasites in free-range backyard chicken flocks in northeast Algeria0 aPrevalence of ectoparasites in freerange backyard chicken flocks cJun-07-2021 a693-7020 v523 a

Free-range backyard chicken breeding is gaining popularity in Algerian rural regions. Due to the natural rearing conditions, chickens are exposed a wide range of microbes and ectoparasites. Currently, little is known about the variety of ectoparasites infesting free-range chickens in Algeria. The present study was conducted over a two year period on 169 backyard chicken, with the aim of identifying and estimating the prevalence of ectoparasites on different body parts of the chickens, and their immediate environments in northeast Algeria. In total, 9943 ectoparasites were identified, and the seven most dominant species were: Menopon gallinae (82.84%), Goniodes dissimilis (15.97%), Menacanthus stramineus (13.60%), Goniocotes gallinae (6.50%), Lipeurus caponis (5.23%), Argas persicus (9.46%) and Dermanyssus gallinae (1.18%). Menopon gallinae was the most frequent isolated ectoparasite throughout the year. Soft ticks Argas persicus were isolated during spring and summer, mainly from crevices and cracks, while the chicken red mite Dermanyssus gallinae was detected in autumn with a low load on bird body parts and a high abundance in nests. These results list the most abundant ectoparasites in backyard chickens, which could facilitate the improvement of rearing management.

10aAlgeria10abackyard chickens10aectoparasite10aPrevalence1 aNahal, Amir1 aRighi, Souad1 aBoucheikhchoukh, Mehdi1 aBenakhla, Ahmed uhttps://hrcak.srce.hr/24901304520nas a2200289 4500008004100000022002500041245006400066210006300130260001600193300001400209490000700223520366800230653001903898653000903917653001703926653001903943100002103962700001803983700002304001700002704024700002804051700002204079700002004101700002104121700002004142856006804162 2020 eng d a0022-2585, 1938-292800aIdentification of Lice Stored in Alcohol Using MALDI-TOF MS0 aIdentification of Lice Stored in Alcohol Using MALDITOF MS cDec-21-2020 a1126-11330 v583 a

Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.

Résumé

Les poux posent des problèmes majeurs de santé publique et vétérinaire, avec des conséquences économiques. Leur identification est essentielle et nécessite le développement d’une stratégie innovante. Le MALDI-TOF MS a récemment été proposé comme un outil rapide, peu coûteux et précis pour l’identification des arthropodes. L’alcool est l’une des méthodes de stockage les plus fréquemment utilisées et permet de conserver les échantillons pendant de longues périodes à température ambiante. Plusieurs études récentes ont rapporté que l’alcool modifie les profils protéiques résultant de l’analyse de la SEP. Après des études préliminaires sur les poux congelés, le but de cette recherche était d’évaluer l’influence de la conservation de l’alcool sur la précision de l’identification des poux par la SEP MALDI-TOF. À cette fin, des poux conservés dans l’alcool pendant des périodes variables ont été soumis à l’analyse de la SEP et les protocoles de préparation des échantillons ont été optimisés. La reproductibilité et la spécificité des spectres de MS obtenus sur ces arthropodes nous ont permis de mettre en œuvre la base de données des spectres de MS de référence (DB) avec les profils protéiques de sept espèces de poux stockés dans l’alcool. Des tests en aveugle ont révélé une identification correcte de 93,9% de Pediculus humanus corporis (Linnaeus, 1758) et de 98,4% des autres espèces de poux collectées sur le terrain. Cette étude a démontré que le MALDI-TOF MS pouvait être utilisé avec succès pour l’identification des poux stockés dans l’alcool pendant différentes durées.

Attached pdf file is preprint copy

10aidentification10alice10aMALDI-TOF MS10astorage method1 aBenyahia, Hanene1 aOuarti, Basma1 aDiarra, Adama, Zan1 aBoucheikhchoukh, Mehdi1 aMeguini, Mohamed, Nadir1 aBehidji, Makhlouf1 aBenakhla, Ahmed1 aParola, Phillipe1 aAlmeras, Lionel uhttps://academic.oup.com/jme/article-abstract/58/3/1126/604259901406nas a2200205 4500008004100000245013800041210006900179520073300248653001300981653000900994653001501003653000901018653001601027100002001043700002001063700002001083700001901103700002001122856005801142 2014 eng d00aPrevalence and seasonal variation of mallophagan species (Phthiraptera) in free-range chickens from rural localities of oran, Algeria0 aPrevalence and seasonal variation of mallophagan species Phthira3 a

A total of 192 free-range chickens was randomly collected from rural localities in the region of Oran (Algeria) and examined to detect the presence of Mallophagan species and their prevalence, mean intensity and seasonal variation. Eight species were recorded, Chelopistes meleagridis, Cuclotogaster heterographus, Goniocotes gallinae, Goniodes dissimilis, Goniodes gigas, Lipeurus caponis, Menacanthus cornutus and Menopon gallinae. Goniocotes gallinae was the most prevalent lice identified (95,31%). The infection rate was higher in males compared to female chickens with a significant difference (P<0,05). The total number of Mallophagan species encountered was higher in winter season than in the other seasons.

10achickens10alice10aMallophaga10aOran10aTraditional1 aMedjouel, Ilyes1 aBenakhla, Ahmed1 aSenouci, Kheira1 aDjelil, Hanene1 aMatallah, Fouzi uhttp://sjournals.com/index.php/SJAs/article/view/120501635nas a2200253 4500008004100000022001300041245016600054210006900220260000900289300001400298490000700312520083400319653001201153653001701165653001301182653001701195653001001212100002001222700002001242700002001262700001901282700002001301856006001321 2013 eng d a1682835600aPrevalence and Distribution of Chewing Lice (Phthiraptera) in Free Range Chickens from the Traditional Rearing System in the Algerian North East, Area of El-Tarf0 aPrevalence and Distribution of Chewing Lice Phthiraptera in Free c2013 a721 - 7250 v123 a

A study was carried out on 144 local chickens (Gallus gallus domesticus), from rural area of El-Tarf to determine the prevalence of chewing lice (Phthiraptera). The overall prevalence rate was 100% in the El- Tarf poultry. Nine different species of chewing lice were identified during this study: Menopon gallinae, Goniocotes gallinae, Lipeurus caponis, Goniodes dissimilis, Goniodes gigas, Menacanthus stramineus, Cuclotogaster heterographus, Menacanthus cornutus and Menacanthus pallidulus. Menopon gallinae (97.2%) was the most prevalent lice identified. The mean intensity of lice in the area of El-Tarf was 172.5 per chicken. Menopon gallinae was the species which has the highest mean intensity of infestation (83.2%). The distribution of nine chewing lice on poultry and their most preferred sites was determined.

10aAlgeria10aChewing lice10achickens10aPhthiraptera10arural1 aMedjouel, Ilyes1 aBenakhla, Ahmed1 aSenouci, Kheira1 aDjelil, Hanene1 aMatallah, Fouzi uhttp://www.scialert.net/abstract/?doi=ijps.2013.721.72503264nas a2200253 4500008004100000022001300041245007900054210006900133260002100202300001400223490000700237520252000244653001002764653002202774653000902796653002602805653001402831100002802845700001702873700002002890700002002910700002002930856006002950 2018 eng d a0972898800aInventory of lice of mammals and farmyard chicken in North-eastern Algeria0 aInventory of lice of mammals and farmyard chicken in Northeaster conline March 201 a386 - 3960 v113 a

Background and Aim: Lice are permanent ectoparasites, extremely specific to their hosts. Their great importance in veterinary medicine remain significant, they can cause their direct pathogenic actions like irritability, dermatitis, anemia, decreased weight gain, and milk production. The purpose of this work was to made the first time an inventory of mammalian lice in North-eastern Algeria.


Materials and Methods: Our survey of lice infestation was conducted on several animal species from five provinces of North-eastern Algeria. A total of 57 cattle, 83 sheep, 77 goats, 111 wild boars, and 63 farmyard chickens were examined. The collection of lice was carried out much more in mammals and chickens during the winter period. Lice were collected either manually or using brushing and kept in flasks containing 70% ethanol. The identification of lice was achieved in the laboratory using a binocular loupe.


Results: Concerning cattle, 63% and 27% of those examined subjects from Souk-Ahras and Guelma study areas, respectively, were carriers of lice. Damalinia bovis was the louse most frequently found on cattle in these two regions. Three other species were identified in Souk-Ahras: Haematopinus eurysternus (25%), Linognathus vituli (10%), and Solenopotes capillatus (5%). Regarding sheep, 39% and 24% of examined animals in Souk-Ahras and Guelma, were carrying lice. Damalinia ovis was the most frequently encountered lice on sheep in both regions. Linognathus ovillus also was identified in Souk-Ahras, representing 0.3% of the collected lice. Concerning goats, 53% and 30% of examined animals in Souk-Ahras and Guelma, were parasitized of lice. Two species of lice were found: Damalinia caprae and Linognathus africanus. For farmyard chickens, 69% and 100% of the farmyard chicken in Souk-Ahras and Mila were parasitized by lice, respectively. Menopon gallinae was the most frequently encountered louse in farmyard chicken in both regions. Eight other species were identified in Mila and four other species only in Souk-Ahras. Finally, 25% and 28% of the wild boars in Annaba and El Tarf were parasitized by lice, respectively. Haematopinus suis was the only species found on wild boars in both regions.

Conclusion: These results are to be taken into account for lice control schemes and louse-borne diseases. Keywords: boars, farmyard chickens, lice, North-eastern Algeria, ruminants.

10aboars10afarmyard chickens10alice10aNorth-eastern Algeria10aruminants1 aMeguini, Mohamed, Nadir1 aRighi, Souad1 aZeroual, Faycal1 aSaidani, Khelaf1 aBenakhla, Ahmed uhttp://www.veterinaryworld.org/Vol.11/March-2018/21.pdf