TY - JOUR T1 - Antigenic characterization of rat louse Polyplax spinulosa JF - Folia Parasitologica (Praha) Y1 - 1990 A1 - Volf,P. A1 - Grubhoffer,L. A1 - Matha,V. SP - 275 EP - 278 KW - animals KW - Antigens/chemistry/immunology KW - Chromatography KW - electrophoresis KW - Immunoblotting KW - Lice Infestations/immunology KW - Lice/immunology KW - Molecular Weight KW - protein KW - rat AB - Number and relative molecular weights of proteins in whole body homogenates of the louse, Polyplax spinulosa were determined using SDS-PAGE. 13 protein components from the total of 28 bands were defined as Con A binding glycoproteins. In immunoblotting sera of lice infested laboratory rats of BN strain specifically recognized 3 major protein components with r.m.w. of 77,000, 105,000 and 230,000 Da and from 6 to 8 minor protein components with r.m.w. of 31,000-180,000 Da. CY - CZECHOSLOVAKIA VL - 37 SN - 0015-5683 IS - 3 N1 - LR: 20031114; JID: 0065750; 0 (Antigens); 0 (Proteins); ppublish ER - TY - JOUR T1 - Ivermectin: its effect on the immune system of rabbits and rats infested with ectoparasites JF - Veterinary immunology and immunopathology Y1 - 1992 A1 - Uhlir,J. A1 - Volf,P. SP - 325 EP - 336 KW - animals KW - Antibodies/blood KW - Blotting, Western KW - ectoparasite KW - electrophoresis KW - enzyme KW - Ivermectin/pharmacology KW - Lice/immunology KW - Lymphocytes/drug effects KW - Mites/immunology KW - Rabbit KW - rat KW - Rodent Diseases/immunology AB - The influence of subcutaneously administered ivermectin on the specific immune response was studied in rabbits infested with mites (Psoroptes cuniculi) and in rats infested with lice (Polyplax spinulosa). A pronounced specific antibody activity and a change in immunoblotting pattern was observed in rabbits after the ivermectin treatment. However, in rats the antibody activity decreased and the profile of specific antibodies, tested by immunoblotting, remained the same as before the treatment. The specific immune response in rabbits artificially immunized with whole-body Psoroptes cuniculi extract was not affected by ivermectin. It was concluded that ivermectin has no direct effect on the immune response of rabbits and rats and that the enhanced immune response in the mite-infested rabbits was caused by the massive release of antigens associated with the synchronous death of the mites. CY - NETHERLANDS VL - 34 SN - 0165-2427 IS - 3-4 N1 - LR: 20031114; JID: 8002006; 0 (Antibodies); 70288-86-7 (Ivermectin); ppublish ER -