A rapid protocol for clearing, staining, and mounting of Arthropoda: Trombiculidae, Pediculidae and Pulicidae

Publication Type:Journal Article
Year of Publication:2021
Authors:P. Philip_Samuel, Govindarajan, R., Krishnamoorthi, R., Venkatesh, A.
Journal:NORTH-WESTERN JOURNAL OF ZOOLOGY
Volume:17
Issue:1
Pagination:1-5
Date Published:June
Type of Article:Open Access Article No.: e201104
Keywords:Arthropoda, clearing, ectoparasites, mounting, staining
Abstract:

This is a rapid protocol proposed after its prolonged usage for the clearing, staining, and mounting of the medically important hematophagous ectoparasites obtained from human and small mammals collected in Tamil Nadu and Kerala, India. During the biodiversity study on ectoparasites, this altered protocol improved significantly the visibility of the major essential taxonomic key characters present on the minute body of the uncharacterized chigger mites, fleas, and lice viewed under phasecontrast microscopy for identification. This modified method will be enormously useful for imparting training, teaching, and research on acarology.

URL:https://biozoojournals.ro/nwjz/content/v17n1/nwjz_e201104_PhilipSamuel.pdf
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